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質量分析用サンプル調製

運用コストの削減

臨床および毒性学ラボでは、質量分析アッセイは増加する一方であり、マニュアル操作によるサンプル調製というワークフローの中の大きなボトルネックへの対応が急務です。Tecanでは、主要な抽出技術への深い理解の下に、高精度のリキッドハンドリングを組合わせてカスタマイズされたワークフローの自動化ソリューションを提供します。

 

関連製品: リキッドハンドリングとオートメーション




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Tab 01 / 加圧式SPE
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加圧式固相抽出

SPEカートリッジは、病院、法医学のための薬物検査が必要な毒性学サービスラボ、生物学的分析/生物学的同等性試験を手掛けるCRO、および食品汚染検査ラボなど、幅広いラボで使用されています。

マニュアル操作でのプロセスは非常に労働集約的であり、担当者はRSIを起こしやすく、日常的に危険な溶剤に曝露しています。人的事故はTATの低下とそれによる検査の更なる反復につながる傾向があり、コストの増加と全体的な効率の低下を招きます。

詳細はこちら




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Tab 02 / アフィニティー精製
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タンパク質のアフィニティ精製

質量分析は、その感度、精度およびPTMの分解能により、バイオマーカー研究で選択される実験ツールとなっています。しかし、従来のMS解析用濃縮法は労働集約的であり、信頼性が低いものでした。

MSIA™(質量分析免疫測定法)Streptavidin EVOマイクロカラムは、様々な種類のサンプルから微量タンパク質を精製し濃縮する能力を備え、次世代のアフィニティーマイクロカラムを代表するものです。




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Tab 03 / カタログ・資料
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カタログ

Easy automation of manual SPE methods using the Freedom EVO positive pressure SPE workstation reduces overall solvent consumption and operator exposure to hazardous solvents, with an intuitive TouchTools™ graphical user interface to minimize the need for user training.

End-to-end sample preparation for mass spectrometry using the Freedom EVO platform. Automate even the most challenging protocols, eliminating the bottleneck of manual sample preparation.

アプリケーションノート

Overcoming the challenges associated with affinity purification methods using the Thermo Scientific MSIA™ workflow on the Freedom EVO® platform. This solution combines robust and high throughput liquid handling with MSIA Streptavidin EVO microcolumns for fast and accurate isolation of target analytes from complex biological matrices.

This workflow combines the Freedom EVO platform with MSIA Streptavidin EVO microcolumns and biotin-conjugated anti-IgG Fc affinity ligands to provide a highly sensitive and reproducible method for the quantitative analysis of human IgG mAbs of different subclasses. All the pre-analytical steps are performed on the Freedom EVO, using the MCA 96 for high throughput processing.

Combining the Freedom EVO platform with the MSIA Streptavidin EVO microcolumns enables the characterization of multiple intact mAbs over a wide dynamic range.

SPE sorbent screening with the Freedom EVO platform and the Strata-X 96-well SPE Method Development (MD) Plate (Phenomenex). This completely automated approach can quickly and efficiently identify the optimal SPE sorbent, as well as providing walkaway routine processing to eliminate manual sample preparation.

Development of an automated hydrolysis and SPE extraction method on a Freedom EVO platform, offering quantitative determination of opioids, cocaine and other metabolites in urine for forensic toxicology.

Tecan Journal記事

Combining the Freedom EVO positive pressure SPE workstation with the Phenomenex Strata-X 96-well SPE Method Development Plates offers a new paradigm in SPE method development for CROs and diagnostic labs, reducing development time from six to seven days to under two hours.

Thermo Fisher Scientific has developed innovative sample processing tools designed to simplify and accelerate analytical workflows. Offering automation-friendly affinity purification for proteins and antibodies, this provides a robust solution for high throughput preclinical research and development.

A Freedom EVO workstation is providing automated sample preparation for LC analysis of vitamins at the Medical Laboratories of the Reinier de Graaf Hospital, generating major time and cost savings and meeting the laboratory’s increasing throughput demands.

A Freedom EVO-based platform for automated antimicrobial susceptibility testing and MALDI-TOF sample preparation is proving beneficial to the Medical Laboratory Bremen, offering enhanced reproducibility compared to manual processes for more accurate results.

A RECIPE for success
[TJ 03/2014]

Tecan and RECIPE have automated analysis of vitamin D and immunosuppressants using a Freedom EVO platform and ClinMass® LC-MSMS Complete Kits, helping to meet the demand for high throughput sample preparation for mass spectrometry.

A Freedom EVO workstation is being used by the University Hospital of Padova to prepare LC-MSMS samples for immunosuppressant drug monitoring in transplant patients.

ADPEN Laboratories has invested in a Freedom EVO liquid handling platform for mass spectrometry sample preparation.

研究用途向けです。