Precision and flexibility in single- to multi-cell and ultra-low volume reagent dispensing.

The Duo Digital Dispenser™ delivers nanoliter-to-picoliter volumes of both reagents and cells, enabling streamlined sample preparation workflows for single-cell and low-input proteomics. Building upon the legacy of the Uno Single Cell Dispenser™, the Duo enhances cell isolation precision down to 7 μm and increases throughput. The system supports surfactant-free fluid handling. In comparative experiments with HEK293 and HEK293-GFP cells, Duo demonstrated comparable dispensing accuracy to Uno, while enabling improved well occupancy and consistent proteomic readouts using a 5-minute bottom-up proteomics protocol. Duo’s open design and software-based plate randomization make it ideal for customizable, label-free or multiplexed proteomics workflows that demand high fidelity and rapid turnaround.

For over a decade, the D300e Digital Dispenser has become a mainstay in laboratories conducting doseresponse studies, drug-synergy experiments, enzyme profiling, and PCR assays where low-volume, noncontact, precision dispensing is required. The D300e substantially reduces workflow length and cost by automating manual workflow steps that take time and waste precious reagents.

The new Duo Digital Dispenser features an upgraded microfluidic cassette that enables direct dispensing of aqueous solutions without requiring surfactants. In contrast to the D300e, which needed surfactants to reliably dispense aqueous fluids, the Duo achieves the same dependable performance without them. This advancement is particularly valuable for cellbased and biochemical assays that are incompatible with surfactant-containing buffers, or when working with molecules whose activity can be affected by surfactants.